Begin by loading a TXT file exported from Äkta's UNICORN ^TM software to visualise a chromatogram. Choose the plot background color, whether to display 260 and/or 280 nm curves, and fit a baseline if needed. Download your plots as PDF, TIFF, or EPS files using the provided 'Download' options. For curve and area color customisation, visit the 'Colors' tab.

This app is specifically designed for chromatogram files from Äkta's UNICORN ^TM software. To visualise chromatograms from other LC manufacturers, reformat them to align with UNICORN ^TM 's output. This involves saving them as TXT files in UTF-16 format and adjusting the file header and column names. An example header suitable for input files can be downloaded below ('Table_header.png'). Additionally, a template file ('Template.xlsx') is available for manual data entry of elution times, intensities, and fraction numbers. Ensure fractions in this file have unique names.

You can fill a restricted area under the 260/280 nm curves, corresponding to particular fractions or elution volume. It is also possible to plot selected fractions under the curve. To do this, choose min/max fraction (max > min) or ml (decimals should be typed with a decimal point, not with a comma, e.i. 1.1) and tick one of the boxes “Highlight fractions/ml on/under the peak”. You can also select the color of the highlighted area under the peak in the “Colors” tab.

It allows you to change the labels on the axes of the plot and zoom in. Type in minimum and maximum x and y (max > min).